《植物生理学报》 2015, 51(11): 1887-1894

盐胁迫对莱茵衣藻光合活性及代谢产物的影响

高翔^1,2, 周维成^1,2, 张凤格^1,2, 陈兰州³, 王高鸿^1,*

¹中国科学院水生生物研究所藻类生物学重点实验室, 武汉430072; ²中国科学院大学, 北京100049; ³武汉大学资源环境学院, 武汉430070

通信作者：王高鸿；E-mail: space@ihb.ac.cn；Tel: 027-68780036

摘　要：

利用单细胞真核模式生物衣藻作为材料, 研究盐胁迫对衣藻细胞生长、光合活性、色素及中性脂肪积累的影响。结果显示, 衣藻细胞生长随盐浓度升高受抑制程度不断增大。盐胁迫下的对数期细胞生长速率、细胞叶绿素a含量和细胞叶绿素b含量都随盐浓度升高而减小。细胞类胡萝卜素含量在低盐胁迫下基本不变, 但在中盐和高盐胁迫中则大幅增高。不同处理的藻细胞中性脂肪积累量都明显上升, 而中盐处理升高的最多。叶绿素荧光动力学参数测定表明盐胁迫抑制电子传递效率和PSII活性。

关键词：莱茵衣藻; 盐胁迫; 色素含量; 光系统II; 中性脂肪

收稿：2015-07-30   修定：2015-10-04

资助：水体污染控制与治理科技重大专项(水专项) (2014ZX07302-003)。

Effect of Salt Stress on Photosynthetic Activity and Metabolites of Chlamydomonas reinhardtii

GAO Xiang^1,2, ZHOU Wei-Cheng^1,2, ZHANG Feng-Ge^1,2, CHEN Lan-Zhou³, WANG Gao-Hong^1,*

¹Key Laboratory of Algae Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; ²University of Chinese Academy of Sciences, Beijing 100049, China; ³College of Resource and Environment Sciences, Wuhan University, Wuhan 430070, China

Corresponding author: WANG Gao-Hong; E-mail: space@ihb.ac.cn; Tel: 027-68780036

Abstract:

In this study model organism Chlamydomonas reinhardtii was used to investigate changes in growth, photosystem II (PSII) activity, pigments and neutral lipids accumulation under different levels of salinity. It was found that the inhibition of salinity on cell growth was depended on salinity levels. The growth rates and the content of chlorophyll a and b of algae cell decreased with the increasing of NaCl concentrations. However the contents of cell carotenoid varied differently as compared to chlorophyll a or b, which remained unchanged in the low salt treatment group but increased significantly in the moderate and high salt stress treatment groups. Additionally, neutral lipids content of cell were significantly increased under salt stress treatment, and which in moderate salinity were highest. Finally the chlorophyll a fluorescence parameters showed that the increased salinity inhibited the electron transduction chain and the activity of PSII.

Key words: Chlamydomonas reinhardtii; salt stress; pigments; PSII; neutral lipids